Part:BBa_K4811006:Design
TMS(GFP1)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 16
Illegal BglII site found at 105
Illegal XhoI site found at 114
Illegal XhoI site found at 150 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
When examining the structure of the TMS from A. Paul and the GFP aptamer from B. Shui it became apparent, that A. Paul had included a linker between the GFP aptamer and the TMS, consisting of GGACAGATCT before the aptamer, and the complementary AGATCTGTCC after the aptamer, although the function of this linker was not specified in their paper. Because the mechanism of repression by GFP on the TMS was not fully understood, the linker was included in the BioBrick.
Source
The part was developed by Avishek Paul during their PHD thesis, see the publication "Modular and Versatile Trans-Encoded Genetic Switches", A. Paul, E. M. Warszawik, M. Loznik, A. J. Boersma, A. Herrmann, Angew. Chem. Int. Ed. 2020, 59, 20328.
For information on the GFP-sensitive aptamer, see the publication "RNA aptamers that functionally interact with green fluorescent protein and its derivatives", B. Shui, A. Ozer, W. Zipfel, N. Sahu, A. Singh, J. T. Lis, H. Shi, M. I. Kotlikoff, Nucleic Acids Res. 2012, 40, e39